Jung Yoo-jin began her entertainment career as a model before making her official acting debut in the 2015 SBS series Heard It Through the Grapevine. That same year, she joined the main cast of OnStyle’s first original drama Because It’s the First Time. In 2016, she starred in KBS2’s Moorim School, which was followed by a supporting role in the hit MBC series W—Two Worlds. In 2017, she became part of the JTBC romance drama Pretty Noona Who Buys Me Food and the SBS romantic comedy Thirty But Seventeen. In 2019, she acted alongside Lee Na-young, Lee Jong-seok, and Wie Ha-joon in tvN’s Romance Is a Bonus Book. She then played a police officer in the crime drama Catch the Ghost, which also aired on tvN. Also in 2019, she appeared in the romance film Tune in for Love. In 2021, she was cast as one of the main characters in JTBC’s Snowdrop.

Jung Yoo-jin attended Dongduk Women’s University.